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one step lumitein uv protein gel stain  (Biotium)


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    Structured Review

    Biotium one step lumitein uv protein gel stain
    (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
    One Step Lumitein Uv Protein Gel Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one+step+lumitein+uv+protein+gel+stain/One-Step+Lumitein+UV+Protein+Gel+Stain/pmc10038098-293-15-21
    Average 91 stars, based on 1 article reviews
    one step lumitein uv protein gel stain - by Bioz Stars, 2026-11
    91/100 stars

    Images

    1) Product Images from "Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii"

    Article Title: Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii

    Journal: Life Science Alliance

    doi: 10.26508/lsa.202201710

    (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
    Figure Legend Snippet: (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.

    Techniques Used: Construct, Purification, Activity Assay, Modification, Positive Control, SDS Page, Silver Staining, Incubation, Staining

    Related Articles

    Silver Staining:

    Article Title: Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii
    Article Snippet: .. Samples were resolved by SDS–PAGE and visualised with the Silver Stain Plus kit (Bio-Rad) or One-Step Lumitein UV Protein Gel Stain (Biotium). ..

    Staining:

    Article Title: Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii
    Article Snippet: .. Samples were resolved by SDS–PAGE and visualised with the Silver Stain Plus kit (Bio-Rad) or One-Step Lumitein UV Protein Gel Stain (Biotium). ..



    Similar Products

    one step lumitein uv protein gel stain  (Biotium)
    91
    Biotium one step lumitein uv protein gel stain
    (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or <t>Lumitein</t> staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.
    One Step Lumitein Uv Protein Gel Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one+step+lumitein+uv+protein+gel+stain/One-Step+Lumitein+UV+Protein+Gel+Stain/pmc10038098-293-15-21
    Average 91 stars, based on 1 article reviews
    one step lumitein uv protein gel stain - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.

    Journal: Life Science Alliance

    Article Title: Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii

    doi: 10.26508/lsa.202201710

    Figure Lengend Snippet: (A) Constructs used in this study. Truncated constructs were used where full-length constructs could not be expressed or purified. (A, B) Reactivity of purified constructs from (A) against the activity-based probe Ub–propargylamine (Ub-PA, 1-h reactions). Asterisk (*): Ub-modified construct. (A, C) Reactivity of purified constructs from (A) against activity-based probe NEDD8-PA (1-h reactions). Asterisk (*): NEDD8-modified construct. (D) Cleavage of precursor pro-NEDD8 by TgOTU DUBs. Human DEN1 is used as a positive control. 1-h reactions were resolved on SDS–PAGE, and pro-NEDD8 cleavage was visualised by silver staining. (E) Activity of TgOTU DUBs against the eight diUb linkage types. (A) Purified constructs in (A) at indicated concentrations were incubated with each diUb linkage type (1.2 μM) and sampled at 0′, 5′, and 30′. Samples were resolved on SDS–PAGE, and diUb/monoUb was visualised by silver staining or Lumitein staining. (E, F) Qualitative heatmap summary based on densitometry of cleavage assays in (E). Data are representative of three independent experiments.

    Article Snippet: Samples were resolved by SDS–PAGE and visualised with the Silver Stain Plus kit (Bio-Rad) or One-Step Lumitein UV Protein Gel Stain (Biotium).

    Techniques: Construct, Purification, Activity Assay, Modification, Positive Control, SDS Page, Silver Staining, Incubation, Staining